Phy

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  • Description: phytase

Gene name phy
Synonyms yodV, yzxA
Essential no
Product 3-phytase
Function untilization of inositol hexakisphosphate (phytate)
MW, pI 41 kDa, 4.936
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours cgeB, yodU
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Phy context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

phosphate metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU19800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to Swiss-Prot)
  • Protein family: msrA Met sulfoxide reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: extracellular (signal peptide) PubMed

Database entries

  • Structure: 1QLG (enzyme from B. amyloliquefaciens, 72% identity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • the gene is not expressed in B. subtilis due to the absene of a functional promoter PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Martha Guerrero-Olazarán, Lilí Rodríguez-Blanco, J Gerardo Carreon-Treviño, Juan A Gallegos-López, José M Viader-Salvadó
Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme.
Appl Environ Microbiol: 2010, 76(16);5601-8
[PubMed:20601512] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Oliwia Makarewicz, Sarah Dubrac, Tarek Msadek, Rainer Borriss
Dual role of the PhoP approximately P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the phyC promoter.
J Bacteriol: 2006, 188(19);6953-65
[PubMed:16980498] [WorldCat.org] [DOI] (P p)

N C Ha, B C Oh, S Shin, H J Kim, T K Oh, Y O Kim, K Y Choi, B H Oh
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states.
Nat Struct Biol: 2000, 7(2);147-53
[PubMed:10655618] [WorldCat.org] [DOI] (P p)

S Roels, R Losick
Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis.
J Bacteriol: 1995, 177(21);6263-75
[PubMed:7592393] [WorldCat.org] [DOI] (P p)