GlnK

• Description: two-component sensor kinase, regulation of the glsA-glnT operon
  
  

Gene name	glnK
Synonyms	ycbA, yzgA
Essential	no
Product	two-component sensor kinase
Function	regulation of the glsA-glnT operon
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	43 kDa, 5.731
Gene length, protein length	1161 bp, 387 aa
Immediate neighbours	glsA, glnL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02440

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of GlnL

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: four transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: GlnK-GlnL, GlnK-TnrA PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P40758

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076] [WorldCat.org] [DOI] (P p)

Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)