AraB

• Description: L-ribulokinase
  
  

Gene name	araB
Synonyms
Essential	no
Product	L-ribulokinase
Function	arabinose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	60 kDa, 5.233
Gene length, protein length	1680 bp, 560 aa
Immediate neighbours	araD, araA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28790

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate (according to Swiss-Prot)

• Protein family: ribulokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3JVP (B. halodurans AraB)

• UniProt: P94524

• KEGG entry: [3]

• E.C. number: 2.7.1.16

Expression and regulation

• Operon: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

• Sigma factor: sigA

• Regulation: repressed by glucose (CcpA)
  • repressed in the absence of arabinose (AraR) PubMed

• Regulatory mechanism: CcpA: transcription repression
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

Isabel Sa-Nogueira, Teresa V Nogueira, Snia Soares, Hermnia de Lencastre
The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression.
Microbiology (Reading): 1997, 143 ( Pt 3);957-969
[PubMed:9084180] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, H de Lencastre
Cloning and characterization of araA, araB, and araD, the structural genes for L-arabinose utilization in Bacillus subtilis.
J Bacteriol: 1989, 171(7);4088-91
[PubMed:2500424] [WorldCat.org] [DOI] (P p)