araN

168

ABC transporter for the uptake of α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (sugar-binding protein)

Locus: BSU_28750

Molecular weight: 48.48 kDa

Isoelectric point: 8.56

Protein length: 433 aa

Gene length: 1302 bp

Function: uptake of α-1,5-arabinooligosaccharides

Product: α-1,5-arabinooligosaccharide ABC transporter (sugar-binding protein)

Essential: no

Synonyms: araN, yseC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

Gene

Coordinates: 2,941,674 → 2,942,975

The protein

Protein family

• Bacterial solute-binding protein 1 family (according to UniProt)

Structure

• 5YSB (PDB) (from Listeria innocua, 28% identity) PubMed
• P94528 (AlphaFold)

Localization

• cell membrane (via AraP-AraQ) PubMed

Expression and Regulation

Operons

• Genes: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

  Description: PubMed

  Regulation

  • induced by arabinose (AraR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • AraR: repression, PubMed, in araR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-B004 (araN::erm), available at the NBRP B. subtilis, Japan
• BKE28750 (ΔaraN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGCGTTACCCCTTCCA,  downstream forward: _UP4_TAGAATCCCATTCAAAAAGT
• BKK28750 (ΔaraN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGCGTTACCCCTTCCA,  downstream forward: _UP4_TAGAATCCCATTCAAAAAGT

References

Loading