PucA

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  • Description: xanthine dehydrogenase

Gene name pucA
Synonyms yurF
Essential no
Product xanthine dehydrogenase
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Purine catabolism, Nucleotides (regulation)
MW, pI 36 kDa, 6.359
Gene length, protein length 990 bp, 330 aa
Immediate neighbours pucB, pucG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PucA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed in the absence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
    • [PucR]]: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References