pucA

168

xanthine dehydrogenase

Locus: BSU_32510

Molecular weight: 36.59 kDa

Isoelectric point: 6.36

Protein length: 330 aa

Gene length: 993 bp

Function: purine utilization

Product: xanthine dehydrogenase

Essential: no

Synonyms: pucA, yurF

Genomic Context

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List of homologs in different organisms, belongs to COG1975 (Galperin et al., 2021)

Gene

Coordinates: 3,339,945 → 3,340,937

The protein

Catalyzed reaction/ biological activity

• H₂O + NAD⁺ + xanthine --> H⁺ + NADH + urate (according to UniProt)
• H₂O + hypoxanthine + NAD⁺ --> H⁺ + NADH + xanthine (according to UniProt)

Structure

• 3ON5 (PDB) (from B. halodurans, 44% identity)
• O32147 (AlphaFold)

Expression and Regulation

Operons

• Genes: pucA-pucB-pucC-pucD-pucE

  Description: PubMed

  Regulation

  • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed

  Regulatory mechanism

  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A965 (yurF::erm), available at the NBRP B. subtilis, Japan
• BKE32510 (ΔpucA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC,  downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT
• BKK32510 (ΔpucA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC,  downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT

References

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