YvcJ

• Description: nucleotide-binding protein
  
  

Gene name	yvcJ
Synonyms
Essential	no
Product	nucleotide-binding P-loop-containing protein
Function	required for the expression of late competence genes
MW, pI	33 kDa, 6.702
Gene length, protein length	885 bp, 295 aa
Immediate neighbours	yvcK, yvcI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34770

Phenotypes of a mutant

reduced competence PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed

• Protein family: UPF0042 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: O06973

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Anne Galinier, University of Marseille, France

References

1. Galinier, A., Haiech, J., Kilhoffer, M.-C., Jaquinod, M., Stülke, J., Deutscher, J., & Martin-Verstraete, I. (1997) The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc. Natl. Acad. Sci. USA 94: 8439-8444. PubMed
2. Görke et al. (2005) YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway. Microbiology 151: 3777-3791. PubMed
3. Luciano , J., E. Foulquier, J.-R. Fantino, A. Galinier, and F. Pompeo (2009) Characterization of YvcJ, a conserved P-loop-containing protein, and Its implication in competence in Bacillus subtilis. J. Bacteriol. 191: 1556-1564-3791. PubMed