SigL

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Gene name sigL
Synonyms
Essential no
Product RNA polymerase sigma-54 factor (sigma-L)
Function utilization of arginin, acetoin and fructose,
required for cold adaptation
Gene expression levels in SubtiExpress: sigL
Interactions involving this protein in SubtInteract: SigL
MW, pI 49,5 kDa, 7.79
Gene length, protein length 1308 bp, 436 amino acids
Immediate neighbours yvfH, yvfG
Sequences Protein DNA DNA_with_flanks
Genetic context
SigL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigL expression.png
















Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

CcpA regulon

The SigL regulon

The gene

Basic information

  • Locus tag: BSU34200

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
  • Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
  • Paralogous protein(s):

Transcription factors activating transcription at SigL-dependent promoters

AcoR, BkdR, LevR, RocR, YplP

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA binding domain (H-T-H motif) (324–343)
    • pron box domain (413–421)
    • 3 x Compositional bias domain (6–21),(32–53),(112–136)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.

Transcription factors activating transcription at SigL-dependent promoters

Expression and regulation

  • Operon: sigL (according to DBTBS)
  • Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

Reviews

Original publications