RoxS

• Description: small RNA, controls expression of ppnKB and sucC-sucD
  
  

Gene name	roxS
Synonyms	rsaE
Essential	no
Product	trans-acting regulatory RNA
Function	control of ppnKB and sucC-sucD expression
Gene length
Immediate neighbours	yizD, yjbH
Gene sequence (+200bp)
Genetic context

Categories containing this gene/protein

ncRNA

This gene is a member of the following regulons

ResD regulon

The RoxS regulon

• ppnKB, sucC-sucD

The gene

Basic information

• Locus tag:

Database entries

• BsubCyc: no entry

• DBTBS entry: no entry

• SubtiList entry: no entry

• Structure:

Additional information

• Similar RNA:

• Interactions:

The RNA

Basic information/ Evolution

• Catalyzed reaction/ biological activity: hybridizes to ppnKB and sucD mRNAs to inhibit their translation and to destabilize them PubMed

Extended information on the RNA

• Kinetic information

• Interactions:
  • binds ppnKB, and sucC-sucD mRNAs PubMed

• Localization

Database entries

• BsubCyc: ?

• Structure:

Expression and regulation

• Operon: roxS PubMed

• Sigma factor:

• Regulation:
  • induced by nitric oxide (NO) (ResD) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed

• Additional information:
  • the roxS RNA is degraded by the joined activities of RNase Y (initial endonucleolytic cleavage) and RNase J1 (processive degradation) PubMed
  • the roxS-ppnKB and roxS-sucC RNA duplexes form a cleavage site for RNase III PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/RNA

Ciaran Condon, Paris