ytdI

168

inorganic polyphosphate/ATP-NAD kinase

Locus: BSU_29540

Molecular weight: 30.10 kDa

Isoelectric point: 6.49

Protein length: 267 aa

Gene length: 804 bp

Function: generation of NADP from NAD

Product: inorganic polyphosphate/ATP-NAD kinase

Essential: no

E.C.: 2.7.1.23

Synonyms: ytdI, ppnKB, nadK2

Genomic Context

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List of homologs in different organisms, belongs to COG0061 (Galperin et al., 2021)

Gene

Coordinates: 3,021,233 → 3,022,036

The protein

Catalyzed reaction/ biological activity

• ATP + NAD⁺ --> ADP + H⁺ + NADP⁺ (according to UniProt)

Protein family

• NAD kinase family (with NadF, according to UniProt)

Structure

• 1YT5 (PDB) (from Thermotoga maritima)\tPubMed
• O34934 (AlphaFold)

Paralogous protein(s)

• NadF

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ytdI

  Description: PubMed

  Additional information

  • half-life of the mRNA: 8.4 min, in a roxS mutant 15 min, in a rny mutant 24 min PubMed

Other regulations

• RoxS: translation inhibition, inhibition of translation and initiation of RNA degradation by Rny and Rnc PubMed translation is inhibited by binding of the RoxS RNA to the mRNA PubMed

Biological materials

Mutant

• MGNA-A109 (ytdI::erm), available at the NBRP B. subtilis, Japan
• BKE29540 (ΔytdI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCCCATTTCCCCTTTG,  downstream forward: _UP4_TAGAAAATAAAAGGACAGGC
• BKK29540 (ΔytdI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCCCATTTCCCCTTTG,  downstream forward: _UP4_TAGAAAATAAAAGGACAGGC

References

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