NagZ

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  • Description: N-acetylglucosaminidase

Gene name nagZ
Synonyms yzbA, ybbD
Essential no
Product N-acetylglucosaminidase
Function cell wall recycling
Gene expression levels in SubtiExpress: nagZ
Metabolic function and regulation of this protein in SubtiPathways:
nagZ
MW, pI 70 kDa, 9.76
Gene length, protein length 1926 bp, 642 aa
Immediate neighbours ybbC, amiE
Sequences Protein DNA DNA_with_flanks
Genetic context
YbbD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NagZ expression.png















Categories containing this gene/protein

cell wall degradation/ turnover, sporulation/ other

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01660

Phenotypes of a mutant

increased autolysis PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: cleaves muropeptides derived from peptidoglycan, but not peptidoglycan itself PubMed
  • Protein family: glycosyl hydrolase 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • secreted (with signal peptide), remains to some extent cell wall-associated PubMed

Database entries

  • KEGG entry: [2]

Additional information

The gene is mis-annotated in KEGG as an ortholog of beta-N-acetylhexosaminidase EC 3.2.1.52. It is marked in MetaCyc as “similar to beta-hexosaminidase”. No EC annotation is available in Swiss-ProtSwiss-Prot.supporting the annotation is available. PubMed

Expression and regulation

  • Regulation:
    • expressed in late exponential and early stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional references: PubMed