namZ

168

exo-β-N-acetylmuramidase

Locus: BSU_01650

Molecular weight: 45.87 kDa

Isoelectric point: 7.37

Protein length: 414 aa

Gene length: 1245 bp

Function: cell wall turnover and recycling

Product: exo-β-N-acetylmuramidase

Essential: no

Synonyms: ybbC, yzbB

Genomic Context

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List of homologs in different organisms, belongs to COG3876 (Galperin et al., 2021)

Gene

Coordinates: 185,194  186,438

The protein

Catalyzed reaction/ biological activity

• exo-lytic cleavage of β-1,4-MurNAc entities from the non-reducing ends of peptidoglycan chains PubMed
• hydrolysis of the peptidoglycan-derived disaccharide MurNAc-β-1,4-GlcNAc PubMed

Protein family

• DUF1343 protein family PubMed

Structure

• 4K05 (PDB) (from Bacteroides fragilis, 43% identity)
• P40407 (AlphaFold)

Expression and Regulation

Operons

• Genes: murQ-murR-murP-amiE-nagZ-namZ

  Description: PubMed

  Regulation

  • expressed in late exponential and early stationary phase PubMed

  Regulatory mechanism

  • MurR: repression, PubMed, in murR regulon

Biological materials

Mutant

• BKE01650 (namZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTTTTTCTCATGTGTCCCT,  downstream forward: _UP4_TAGAACATAAGCATCGTATG
• BKK01650 (namZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTTTTTCTCATGTGTCCCT,  downstream forward: _UP4_TAGAACATAAGCATCGTATG

References

Reviews

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Original Publications

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