CitG

• Description: fumarase
  
  

Gene name	citG
Synonyms
Essential	no
Product	fumarate hydratase
Function	TCA cycle
Gene expression levels in SubtiExpress: citG
Interactions involving this protein in SubtInteract: CitG
Metabolic function and regulation of this protein in SubtiPathways: citG
MW, pI	50 kDa, 5.475
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	yuxN, yvzF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, most abundant proteins

The gene

Basic information

• Locus tag: BSU33040

Database entries

• BsubCyc: BSU33040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate = fumarate + H₂O (according to Swiss-Prot)

• Protein family: Fumarase subfamily (according to Swiss-Prot)

• Paralogous protein(s): AnsB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • Mdh-CitG PubMed
  • CitG-CitB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU33040

• Structure: 3R6Q (aspartase from Bacillus sp. YM55-1, 43% identity, 77% similarity) PubMed

• UniProt: P07343

• KEGG entry: [3]

• E.C. number: 4.2.1.2

Expression and regulation

• Operon: citG PubMed

• Expression browser: citG PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2427 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 9880 PubMed

Biological materials

• Mutant:
  • GP718 (spec), available in Jörg Stülke's lab

• Expression vector:
  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)

• lacZ fusion:
  • pGP387 (in pAC7), available in Jörg Stülke's lab

• GFP fusion: GP1433 (spc, based on pGP1870), available in the Stülke lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

• FLAG-tag construct:
  • GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab