LipA

• Description: trigger enzyme: lipoic acid synthase and regulator of comE operon expression
  
  

Gene name	lipA
Synonyms	yutB
Essential	Yes PubMed
Product	trigger enzyme: lipoic acid synthase
Function	lipid metabolism, required for the synthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: lipA
Metabolic function and regulation of this protein in SubtiPathways: LipA
MW, pI	33 kDa, 8.251
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	yutC, lytH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, trigger enzyme

The gene

Basic information

• Locus tag: BSU32330

Phenotypes of a mutant

reduced transformation efficiency PubMed, strong inhibition of growth on minimal media PubMed

Database entries

• BsubCyc: BSU32330

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H₂O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed

• Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU32330

• Structure:

• UniProt: O32129
• KEGG entry: [2]

• E.C. number: 2.8.1.8 3.1.1.3]

Expression and regulation

• Operon:

• Expression browser: lipA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Natalia Martin, Esteban Lombardía, Silvia G Altabe, Diego de Mendoza, María C Mansilla
A lipA (yutB) mutant, encoding lipoic acid synthase, provides insight into the interplay between branched-chain and unsaturated fatty acid biosynthesis in Bacillus subtilis.
J Bacteriol: 2009, 191(24);7447-55
[PubMed:19820084] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)