ComGA
- Description: late competence protein, traffic ATPase, binds and transports transforming DNA
Gene name | comGA |
Synonyms | |
Essential | no |
Product | traffic ATPase |
Function | genetic competence |
Gene expression levels in SubtiExpress: comGA | |
Interactions involving this protein in SubtInteract: ComGA | |
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
MW, pI | 40 kDa, 8.893 |
Gene length, protein length | 1068 bp, 356 aa |
Immediate neighbours | comGB, corA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
genetic competence, membrane proteins, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24730
Phenotypes of a mutant
Database entries
- BsubCyc: BSU24730
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binds and transports transforming DNA
- Protein family: GSP E family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU24730
- Structure:
- UniProt: P25953
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- repressed by casamino acids PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159]
[WorldCat.org]
[DOI]
(P p)
Original publications
Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742]
[WorldCat.org]
[DOI]
(I p)
Kenneth Briley, Angella Dorsey-Oresto, Peter Prepiak, Miguel J Dias, Jessica M Mann, David Dubnau
The secretion ATPase ComGA is required for the binding and transport of transforming DNA.
Mol Microbiol: 2011, 81(3);818-30
[PubMed:21707789]
[WorldCat.org]
[DOI]
(I p)
Kenneth Briley, Peter Prepiak, Miguel J Dias, Jeanette Hahn, David Dubnau
Maf acts downstream of ComGA to arrest cell division in competent cells of B. subtilis.
Mol Microbiol: 2011, 81(1);23-39
[PubMed:21564336]
[WorldCat.org]
[DOI]
(I p)
Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J Bacteriol: 2011, 193(7);1633-42
[PubMed:21278288]
[WorldCat.org]
[DOI]
(I p)
Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974]
[WorldCat.org]
[DOI]
(P p)
Jeanette Hahn, Berenike Maier, Bert Jan Haijema, Michael Sheetz, David Dubnau
Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis.
Cell: 2005, 122(1);59-71
[PubMed:16009133]
[WorldCat.org]
[DOI]
(P p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663]
[WorldCat.org]
[DOI]
(P p)
B J Haijema, J Hahn, J Haynes, D Dubnau
A ComGA-dependent checkpoint limits growth during the escape from competence.
Mol Microbiol: 2001, 40(1);52-64
[PubMed:11298275]
[WorldCat.org]
[DOI]
(P p)
Y S Chung, F Breidt, D Dubnau
Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis.
Mol Microbiol: 1998, 29(3);905-13
[PubMed:9723928]
[WorldCat.org]
[DOI]
(P p)
Y S Chung, D Dubnau
All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis.
J Bacteriol: 1998, 180(1);41-5
[PubMed:9422590]
[WorldCat.org]
[DOI]
(P p)
D van Sinderen, A ten Berge, B J Hayema, L Hamoen, G Venema
Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis.
Mol Microbiol: 1994, 11(4);695-703
[PubMed:8196543]
[WorldCat.org]
[DOI]
(P p)
M Albano, R Breitling, D A Dubnau
Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon.
J Bacteriol: 1989, 171(10);5386-404
[PubMed:2507524]
[WorldCat.org]
[DOI]
(P p)