LipA

• Description: trigger enzyme: lipoic acid synthase and regulator of comE operon expression
  
  

Gene name	lipA
Synonyms	yutB
Essential	Yes PubMed
Product	trigger enzyme: lipoic acid synthase
Function	lipid metabolism, required for the synthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: lipA
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	33 kDa, 8.251
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	yutC, lytH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, trigger enzyme

The gene

Basic information

• Locus tag: BSU32330

Phenotypes of a mutant

reduced transformation efficiency PubMed, strong inhibition of growth on minimal media PubMed

Database entries

• BsubCyc: BSU32330

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H₂O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed

• Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU32330

• Structure:

• UniProt: O32129
• KEGG entry: [2]

• E.C. number: 2.8.1.8 3.1.1.3]

Expression and regulation

• Operon:

• Expression browser: lipA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: