CysK

From SubtiWiki
Revision as of 16:23, 5 March 2014 by Jstuelk (talk | contribs)
Jump to: navigation, search
Gene name cysK
Synonyms
Essential no
Product trigger enzyme: cysteine synthetase A
Function biosynthesis of cysteine, control of CymR activity
Gene expression levels in SubtiExpress: cysK
Interactions involving this protein in SubtInteract: CysK
Metabolic function and regulation of this protein in SubtiPathways:
cysK
MW, pI 32 kDa, 5.492
Gene length, protein length 924 bp, 308 aa
Immediate neighbours yacD, pabB
Sequences Protein DNA DNA_with_flanks
Genetic context
CysK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysK expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control, trigger enzyme, most abundant proteins

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU00730

Phenotypes of a mutant

constitutive expression of the cysH-cysP-sat-cysC-ylnD-ylnE-ylnF operon, auxotrophic for cysteine

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + H2S = L-cysteine + acetate (according to Swiss-Prot)
  • Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
  • Interactions:
    • CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

Database entries

  • Structure: 1Y7L (from Haemophilus influenzae, 39% identity, 52% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR) PubMed
  • Regulatory mechanism:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References