ComEA

• Description: membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake
  
  

Gene name	comEA
Synonyms	comD
Essential	no
Product	membrane DNA receptor
Function	genetic competence
Gene expression levels in SubtiExpress: comEA
Interactions involving this protein in SubtInteract: ComEA
Metabolic function and regulation of this protein in SubtiPathways: comEA
MW, pI	21 kDa, 4.824
Gene length, protein length	615 bp, 205 aa
Immediate neighbours	comEB, comER
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon, ComN regulon

The gene

Basic information

• Locus tag: BSU25590

Phenotypes of a mutant

• strongly reduced transformation rate PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • extracellular DNA-binding domain

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • ComEC-ComEA PubMed

• Localization:
  • cell membrane, no distinct position PubMed
  • integral membrane protein PubMed

Database entries

• Structure: 2DUY (from Thermus thermophilus hb8, 37% identity, 60% similarity)

• UniProt: P39694

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comEA-comEB-comEC PubMed

• Expression browser: comEA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • post-translational control by ComN PubMed
  • yutB is required for transcription PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)