PanB

• Description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
  
  

Gene name	panB
Synonyms
Essential	no
Product	3-methyl-2-oxobutanoate hydroxymethyltransferase
Function	biosynthesis of coenzyme A
Gene expression levels in SubtiExpress: panB
Metabolic function and regulation of this protein in SubtiPathways: Coenzyme A
MW, pI	29 kDa, 5.282
Gene length, protein length	831 bp, 277 aa
Immediate neighbours	panC, birA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU22430

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H₂O = tetrahydrofolate + 2-dehydropantoate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3EZ4 (from Burkholderia pseudomallei, 44% identity, 64% similarity)

• UniProt: P52996

• KEGG entry: [2]

• E.C. number: 2.1.2.11

Expression and regulation

• Operon:

• Expression browser: panB PubMed

• Sigma factor:

• Regulation:
  • induced upon fatty acid biosynthesis inhibition PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: PA126 (panB::cat), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michaela Wenzel, Malay Patra, Dirk Albrecht, David Y-K Chen, K C Nicolaou, Nils Metzler-Nolte, Julia E Bandow
Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies.
Antimicrob Agents Chemother: 2011, 55(6);2590-6
[PubMed:21383089] [WorldCat.org] [DOI] (I p)