Tpi

• Description: triose phosphate isomerase, glycolytic/ gluconeogenic enzyme
  
  

Gene name	tpi
Synonyms	tpiA
Essential	no
Product	triosephosphate isomerase
Function	enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: tpi
Interactions involving this protein in SubtInteract: Tpi
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	26,9 kDa, 4.79
Gene length, protein length	759 bp, 253 amino acids
Immediate neighbours	pgm, pgk
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

• Locus tag: BSU33920

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)

• Protein family: triosephosphate isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-213 PubMed

• Cofactor(s):

• Effectors of protein activity: inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed

• Interactions:
  • Tkt-Tpi PubMed

• Localization:
  • cytoplasm PubMed

Database entries

• Structure: 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI

• UniProt: P27876

• KEGG entry: [3]

• E.C. number: 5.3.1.1

Additional information

• extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpiA-pgm-eno PubMed
  • pgk-tpiA-pgm-eno PubMed

• Expression browser: tpi PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (3.3 fold) PubMed
  • cggR: induced by glycolytic substrates CggR PubMed
  • pgk: constitutive PubMed

• Regulatory mechanism: transcription repression by CggR PubMed

• Additional information:

Biological materials

• Mutant: GP700 (cat), available in Stülke lab

• Expression vector:
  • pGP394 (N-terminal His-tag, in pWH844), available in Stülke lab
  • pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Stülke lab
  • pGP1511 (expression in B. subtilis, in pBQ200), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: