Sfp/1

• Description: 4'-phosphopantetheinyl transferase (surfactin synthetase-activating enzyme), inactive pseudogene in strain 168
  
  

Gene name	sfp/1
Synonyms	sfp
Essential	no
Product	4'-phosphopantetheinyl transferase
Function	phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits of surfactin synthetase (SrfAA-SrfAB-SrfAC) and AcpK
MW, pI	- , -
Gene length, protein length	168 bp, -
Immediate neighbours	ycxD, sfp/2
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

miscellaneous metabolic pathways, biofilm formation, biosynthesis of antibacterial compounds, pseudogenes

The gene

Basic information

• Locus tag: BSU03569

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• This gene is a pseudogene in B. subtilis 168 due to a mutation that resulted in an internal frameshift. The second part of the pseudogene is sfp/2.
• Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CoA + apo-[peptidyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] (according to Swiss-Prot)

• Protein family: Gsp/sfp/hetI/acpT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1QR0 (complex with CoA)

• Swiss prot entry:

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: sfp/1 PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)