ArgG

• Description: argininosuccinate synthase, reversible
  
  

Gene name	argG
Synonyms
Essential	no
Product	argininosuccinate synthase, reversible
Function	biosynthesis of arginine
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	44 kDa, 5.028
Gene length, protein length	1209 bp, 403 aa
Immediate neighbours	argH, moaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU29450

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: S-cysteinylation after diamide stress (C187) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1KH1 (from Thermus thermophilus, 44% identity, 65% similarity) PubMed

• UniProt: O34347

• KEGG entry: [2]

• E.C. number: 6.3.4.5

Expression and regulation

• Operon: argG-argH

• Expression browser: argG PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC)

• Regulatory mechanism:
  • AhrC: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: