argH

168

argininosuccinate lyase

locus: BSU_29440

Molecular weight: 51.76 kDa

pI: 4.86

Protein length: 461 aa

Gene length: 1386 bp

function: biosynthesis of arginine

product: argininosuccinate lyase

essential: no

ec: 4.3.2.1

synonyms: argH

List of homologs in different organisms, belongs to COG0165 (Galperin et al., 2021)

Gene

Coordinates: 3,011,751  3,013,136

The protein

Catalyzed reaction/ biological activity

• 2-(N^ω-L-arginino)succinate --> fumarate + L-arginine (according to UniProt)

Protein family

• [wiki|lyase 1 family] (according to UniProt)

Structure

• [PDB|1TJ7] (from ''Escherichia coli'', 47% identity, 64% similarity) [Pubmed|15502303]
• [AF|O34858]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]-[gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH]

  description: [Pubmed|22383849]

  regulation

  • repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC])
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]

  regulatory mechanism

  • [protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: repression, in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]

Biological materials

Mutant

• GP1688 Δ([gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]-[gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH])::''aphA3'', available in [wiki|Jörg Stülke]'s lab
• GP3712 ([gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH]::aphA3 trpC2) available in the [wiki|Stülke] lab
• BKE29440 ([gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29440 BGSC] and in [wiki|Jörg Stülke]'s lab GP3925,  [Pubmed|28189581], upstream reverse: _UP1_AAATCGGCCTCCCCAAAGCT,  downstream forward: _UP4_TAAAATTTCCTTCATAAAAT
• BKK29440 ([gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAATCGGCCTCCCCAAAGCT,  downstream forward: _UP4_TAAAATTTCCTTCATAAAAT

Expression vectors

• pGP3764: expression of Strep-''argH'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP3779: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab

References

• 12107147,21821766,22383849,38832794