Pgk

• Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme
  
  

Gene name	pgk
Synonyms
Essential	yes
Product	Phosphoglycerate kinase
Function	enzyme in glycolysis/ gluconeogenesis
MW, pI	42,0 kDa, 4.77
Gene length, protein length	1182 bp, 394 amino acids
Immediate neighbours	gapA, tpi
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates: 3479231 - 3480412

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate

• Protein family: phosphoglycerate kinase family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • nucleotide binding domain (ATP) (350–353)
  • 2x substrate binding domain (21–23), (59–62)

• Modification: phosphorylation PubMed,

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Pgk-GapA

• Localization: Cytoplasm PubMed

Database entries

• Structure: Geobacillus stearothermophilus NCBI

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpiA-pgm-eno
  • cggR-gapA

• Sigma factor: SigA

• Regulation: neg. regulated by CggR PubMed, induced by sugar

• Regulatory mechanism: repressor CggR PubMed

• Additional information:

References

1. Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Mol Microbiol 41, 409-422.PubMed