RecA

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  • Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)

Gene name recA
Synonyms recE
Essential no
Product multifunctional protein involved in homologous
recombination and DNA repair (LexA-autocleavage)
Function DNA repair/ recombination
Interactions involving this protein in SubtInteract: RecA
MW, pI 37 kDa, 4.883
Gene length, protein length 1041 bp, 347 aa
Immediate neighbours cinA, pbpX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RecA context.gif
This image was kindly provided by SubtiList




Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon, LexA regulon

The gene

Basic information

  • Locus tag: BSU16940

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

RecA filaments are dismantled from DNA by PcrA PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • RecA stimulates ssDNA phosphorylase activity of PnpA PubMed
  • Protein family: recA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser-2 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1U94 (RecA from E. coli, 62% identity, 86% similarity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by DNA damage (LexA) PubMed
    • induced by conditions that trigger development of genetic competence (ComK) PubMed
  • Additional information:

Biological materials

  • Mutant: IRN444 (cat), available in the Stülke lab
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

Your additional remarks

References

Reviews


Original publications

Additional publications: PubMed