NadC

• Description: nicotinate-nucleotide diphosphorylase (carboxylating)
  
  

Gene name	nadC
Synonyms	yrxB
Essential	no
Product	nicotinate-nucleotide diphosphorylase (carboxylating)
Function	NAD biosynthesis
MW, pI	31 kDa, 5.18
Gene length, protein length	867 bp, 289 aa
Immediate neighbours	nadA, nadB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

• Locus tag: BSU27860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nicotinate D-ribonucleotide + diphosphate + CO₂ = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: nadC/modD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39666

• KEGG entry: [3]

• E.C. number: 2.4.2.19

Expression and regulation

• Operon: nadB-nadC-nadA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of nicotinic acid (NadR) PubMed

• Regulatory mechanism:
  • NadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.
J Bacteriol: 1993, 175(5);1423-32
[PubMed:8444804] [WorldCat.org] [DOI] (P p)