ThdF
- Description: GTP-binding protein, putative tRNA modification GTPase
| Gene name | thdF |
| Synonyms | |
| Essential | no |
| Product | putative tRNA modification GTPase |
| Function | tRNA modification |
| MW, pI | 50 kDa, 4.643 |
| Gene length, protein length | 1377 bp, 459 aa |
| Immediate neighbours | gidA, jag |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
translation, GTP-binding proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU41020
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: MnmE subfamily (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: GidA-ThdF (suggested by interaction of E. coli counterparts PubMed and by co-localization on the chromosome)
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P25811
- KEGG entry: [3]
- E.C. number:
Additional information
Information on the corresponding protein from E. coli: EcoCyc MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
Your additional remarks
References
