Nfo

• Description: type IV apurinic/apyrimidinic endonuclease
  
  

Gene name	nfo
Synonyms	yqfS
Essential	no
Product	type IV apurinic/apyrimidinic endonuclease
Function	repair of oxidative DNA damage in spores
MW, pI	32 kDa, 5.371
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	yqfT, cshB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU25130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to Swiss-Prot)

• Protein family: AP endonuclease 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54476

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nfo-yqfU PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

The gene yqfT is located between nfo and yqfU, but is transcribed in the opposite direction.

Biological materials

• Mutant: available in Mario Pedraza-Reyes' lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Marcelo Barraza-Salas, Juan R Ibarra-Rodríguez, Silvia J Mellado, José M Salas-Pacheco, Peter Setlow, Mario Pedraza-Reyes
Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores.
FEMS Microbiol Lett: 2010, 302(2);159-65
[PubMed:19930460] [WorldCat.org] [DOI] (I p)

Juan R Ibarra, Alma D Orozco, Juan A Rojas, Karina López, Peter Setlow, Ronald E Yasbin, Mario Pedraza-Reyes
Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores.
J Bacteriol: 2008, 190(6);2031-8
[PubMed:18203828] [WorldCat.org] [DOI] (I p)

José M Salas-Pacheco, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage.
J Bacteriol: 2005, 187(21);7374-81
[PubMed:16237020] [WorldCat.org] [DOI] (P p)

José M Salas-Pacheco, Norma Urtiz-Estrada, Guadalupe Martínez-Cadena, Ronald E Yasbin, Mario Pedraza-Reyes
YqfS from Bacillus subtilis is a spore protein and a new functional member of the type IV apurinic/apyrimidinic-endonuclease family.
J Bacteriol: 2003, 185(18);5380-90
[PubMed:12949090] [WorldCat.org] [DOI] (P p)

Norma Urtiz-Estrada, José M Salas-Pacheco, Ronald E Yasbin, Mario Pedraza-Reyes
Forespore-specific expression of Bacillus subtilis yqfS, which encodes type IV apurinic/apyrimidinic endonuclease, a component of the base excision repair pathway.
J Bacteriol: 2003, 185(1);340-8
[PubMed:12486072] [WorldCat.org] [DOI] (P p)