Tpi

• Description: catalyzes the transformation of glycerinaldehyd 3-phosphate to dihydroxyactonphosphate and the reverse reaktion
  
  

Gene name	tpi
Synonyms	tpiA
Essential	yes
Product	Triosephosphate isomerase
Function	isomerase
MW, pI	26,9 kDa, 4.79
Gene length, protein length	759 bp, 253 amino acids
Immediate neighbours	pgk, pgm
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = glycerone phosphate

• Protein family: triosephosphate isomerase family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation (STY) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Pgm-TpiA

• Localization: cytoplasm PubMed

Database entries

• Structure: complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpiA-pgm-eno
  • cggR-gapA

• Sigma factor: SigA

• Regulation: neg. regulated by CggR PubMed, induced by sugar

• Regulatory mechanism: repressor CggR PubMed

• Additional information:

References

1. Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Mol Microbiol 41, 409-422.PubMed