Epr

• Description: minor extracellular serine protease, involved in control of swarming motility PubMed
  
  

Gene name	epr
Synonyms	ipa-15r
Essential	no
Product	minor extracellular serine protease
Function	protein degradation
MW, pI	69 kDa, 9.875
Gene length, protein length	1935 bp, 645 aa
Immediate neighbours	ywbA, sacX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase S8 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P16396

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epr PubMed

• Sigma factor: SigD PubMed

• Regulation:
  • repressed by SinR PubMed
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • activation by DegU, not activated by DegU-P PubMed
  • SinR: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Monica Gupta, Kestur Krishnamurthy Rao
Epr plays a key role in DegU-mediated swarming motility of Bacillus subtilis.
FEMS Microbiol Lett: 2009, 295(2);187-94
[PubMed:19416356] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, Madhulika Dixit, K Krishnamurthy Rao
ScoC and SinR negatively regulate epr by corepression in Bacillus subtilis.
J Bacteriol: 2006, 188(17);6425-8
[PubMed:16923912] [WorldCat.org] [DOI] (P p)

Madhulika Dixit, Charuta S Murudkar, K Krishnamurthy Rao
Epr is transcribed from a final sigma(D) promoter and is involved in swarming of Bacillus subtilis.
J Bacteriol: 2002, 184(2);596-9
[PubMed:11751842] [WorldCat.org] [DOI] (P p)

A Sloma, A Ally, D Ally, J Pero
Gene encoding a minor extracellular protease in Bacillus subtilis.
J Bacteriol: 1988, 170(12);5557-63
[PubMed:3142851] [WorldCat.org] [DOI] (P p)