GlsA

• Description: glutaminase, low affinity for glutamine
  
  

Gene name	glsA
Synonyms	ybgJ
Essential	no
Product	glutaminase
Function	glutamine degradation
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	36 kDa, 5.315
Gene length, protein length	981 bp, 327 aa
Immediate neighbours	glnT, glnK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: l-glutamine + H₂O = L-glutamate + NH₃ (according to Swiss-Prot)

• Protein family: glutaminase family (according to Swiss-Prot)

• Paralogous protein(s): YlaM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BRM

• UniProt: O31465

• KEGG entry: [3]

• E.C. number: 3.5.1.2

Expression and regulation

• Operon: glsA-glnT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of glutamine (GlnL) PubMed

• Regulatory mechanism:
  • GlnL: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Greg Brown, Alex Singer, Michael Proudfoot, Tatiana Skarina, Youngchang Kim, Changsoo Chang, Irina Dementieva, Ekaterina Kuznetsova, Claudio F Gonzalez, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2008, 47(21);5724-35
[PubMed:18459799] [WorldCat.org] [DOI] (I p)

Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196] [WorldCat.org] [DOI] (P p)