BioI

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  • Description: cytochrome P450 enzyme

Gene name bioI
Synonyms
Essential no
Product cytochrome P450 enzyme
Function biosynthesis of biotin
MW, pI 44 kDa, 6.2
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours ytbQ, bioB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BioI context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU30190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: cytochrome P450 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Rachel J Lawson, David Leys, Michael J Sutcliffe, Carol A Kemp, Myles R Cheesman, Susan J Smith, John Clarkson, W Ewen Smith, Ihtshamul Haq, John B Perkins, Andrew W Munro
Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis.
Biochemistry: 2004, 43(39);12410-26
[PubMed:15449931] [WorldCat.org] [DOI] (P p)

Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930] [WorldCat.org] [DOI] (P p)

Amanda J Green, Andrew W Munro, Myles R Cheesman, Graeme A Reid, Claes von Wachenfeldt, Stephen K Chapman
Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI.
J Inorg Biochem: 2003, 93(1-2);92-9
[PubMed:12538057] [WorldCat.org] [DOI] (P p)

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)