TatAY

• Description: component of the TatAY-TatCY twin-arginine translocase
  
  

Gene name	tatAY
Synonyms	ydiI
Essential	no
Product	component of the twin-arginine translocation pathway
Function	TAT protein secretion
Gene expression levels in SubtiExpress: tatAY
Interactions involving this protein in SubtInteract: TatAY
Metabolic function and regulation of this protein in SubtiPathways: TatAY
MW, pI	5 kDa, 6.558
Gene length, protein length	171 bp, 57 aa
Immediate neighbours	rex, tatCY
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, membrane proteins

The gene

Basic information

• Locus tag: BSU05980

Database entries

• BsubCyc: BSU05980

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • secretion of EfeB and of YkuE
  • delivers QcrA to the membrane PubMed

• Protein family: tatA/E family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • TatAY-TatCY PubMed
  • WprA-TatAY PubMed
  • TatAY-TatAD PubMed
  • TatAC-TatAY PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU05980

• Structure:

• UniProt: O05522

• KEGG entry: [2]

• E.C. number:

Additional information

• TatAY-TatCY-dependent export of YkuE and YwbN requires a functional WprA PubMed

Expression and regulation

• Operon:
  • ydiG-rex-tatAY-tatCY PubMed
  • tatAY-tatCY PubMed

• Expression browser: tatAY PubMed

• Sigma factor:

• Regulation: constitutitvely expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Kenneth Cline
Mechanistic Aspects of Folded Protein Transport by the Twin Arginine Translocase (Tat).
J Biol Chem: 2015, 290(27);16530-8
[PubMed:25975269] [WorldCat.org] [DOI] (I p)

Vivianne J Goosens, Carmine G Monteferrante, Jan Maarten van Dijl
The Tat system of Gram-positive bacteria.
Biochim Biophys Acta: 2014, 1843(8);1698-706
[PubMed:24140208] [WorldCat.org] [DOI] (P p)

Tracy Palmer, Ben C Berks
The twin-arginine translocation (Tat) protein export pathway.
Nat Rev Microbiol: 2012, 10(7);483-96
[PubMed:22683878] [WorldCat.org] [DOI] (I e)

Original publications

Vivianne J Goosens, Alba De-San-Eustaquio-Campillo, Rut Carballido-López, Jan Maarten van Dijl
A Tat ménage à trois--The role of Bacillus subtilis TatAc in twin-arginine protein translocation.
Biochim Biophys Acta: 2015, 1853(10 Pt A);2745-53
[PubMed:26239117] [WorldCat.org] [DOI] (P p)

Roshani Patel, Cvetelin Vasilev, Daniel Beck, Carmine G Monteferrante, Jan Maarten van Dijl, C Neil Hunter, Corinne Smith, Colin Robinson
A mutation leading to super-assembly of twin-arginine translocase (Tat) protein complexes.
Biochim Biophys Acta: 2014, 1843(9);1978-86
[PubMed:24875903] [WorldCat.org] [DOI] (P p)

Vivianne J Goosens, Carmine G Monteferrante, Jan Maarten van Dijl
Co-factor insertion and disulfide bond requirements for twin-arginine translocase-dependent export of the Bacillus subtilis Rieske protein QcrA.
J Biol Chem: 2014, 289(19);13124-31
[PubMed:24652282] [WorldCat.org] [DOI] (I p)

Vivianne J Goosens, Andreas Otto, Corinna Glasner, Carmine C Monteferrante, René van der Ploeg, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
Novel twin-arginine translocation pathway-dependent phenotypes of Bacillus subtilis unveiled by quantitative proteomics.
J Proteome Res: 2013, 12(2);796-807
[PubMed:23256564] [WorldCat.org] [DOI] (I p)

Carmine G Monteferrante, Calum MacKichan, Elodie Marchadier, Maria-Victoria Prejean, Rut Carballido-López, Jan Maarten van Dijl
Mapping the twin-arginine protein translocation network of Bacillus subtilis.
Proteomics: 2013, 13(5);800-11
[PubMed:23180473] [WorldCat.org] [DOI] (I p)

Carmine G Monteferrante, Marcus Miethke, René van der Ploeg, Corinna Glasner, Jan Maarten van Dijl
Specific targeting of the metallophosphoesterase YkuE to the bacillus cell wall requires the twin-arginine translocation system.
J Biol Chem: 2012, 287(35);29789-800
[PubMed:22767609] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

René van der Ploeg, James P Barnett, Nishi Vasisht, Vivianne J Goosens, Dierk C Pöther, Colin Robinson, Jan Maarten van Dijl
Salt sensitivity of minimal twin arginine translocases.
J Biol Chem: 2011, 286(51);43759-43770
[PubMed:22041895] [WorldCat.org] [DOI] (I p)

René van der Ploeg, Ulrike Mäder, Georg Homuth, Marc Schaffer, Emma L Denham, Carmine G Monteferrante, Marcus Miethke, Mohamed A Marahiel, Colin R Harwood, Theresa Winter, Michael Hecker, Haike Antelmann, Jan Maarten van Dijl
Environmental salinity determines the specificity and need for Tat-dependent secretion of the YwbN protein in Bacillus subtilis.
PLoS One: 2011, 6(3);e18140
[PubMed:21479178] [WorldCat.org] [DOI] (I e)

Robyn T Eijlander, Magdalena A Kolbusz, Erwin M Berendsen, Oscar P Kuipers
Effects of altered TatC proteins on protein secretion efficiency via the twin-arginine translocation pathway of Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 6);1776-1785
[PubMed:19383693] [WorldCat.org] [DOI] (P p)

Jan D H Jongbloed, Ulrike Grieger, Haike Antelmann, Michael Hecker, Reindert Nijland, Sierd Bron, Jan Maarten van Dijl
Two minimal Tat translocases in Bacillus.
Mol Microbiol: 2004, 54(5);1319-25
[PubMed:15554971] [WorldCat.org] [DOI] (P p)