tatAY

168

component of the TatAY-TatCY twin-arginine translocase

Locus: BSU_05980

Molecular weight: 5.93 kDa

Isoelectric point: 6.56

Protein length: 57 aa

Gene length: 174 bp

Function: TAT protein secretion

Product: component of the twin-arginine translocation pathway

Essential: no

Synonyms: tatAY, ydiI

Genomic Context

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List of homologs in different organisms, belongs to COG1826 (Galperin et al., 2021)

Gene

Coordinates: 647,760  647,933

The protein

Catalyzed reaction/ biological activity

• secretion of EfeB and of YkuE
• delivers QcrA to the membrane PubMed

Protein family

• TatA/E family (with TatAC and TatAD, according to UniProt)

Structure

• 2L16 (PDB) (TatAD, 60% identity) PubMed
• O05522 (AlphaFold)

Paralogous protein(s)

• TatAC, TatAD

Localization

• cell membrane (according to Swiss-Prot)

Additional information

• TatAY-TatCY-dependent export of YkuE and EfeB requires a functional WprA PubMed

Expression and Regulation

Operons

• Genes: tatAY-tatCY

  Description: PubMed

  Regulation

  • constitutitvely expressed PubMed

• Genes: ydiG-rex-tatAY-tatCY

  Description: PubMed

  Regulation

  • constitutitvely expressed PubMed
  • the mRNA is processed between rex and tatAY by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Biological materials

Mutant

• MGNA-C202 (ydiI::erm), available at the NBRP B. subtilis, Japan
• BKE05980 (tatAY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTGGGCTCCTCCTTT,  downstream forward: _UP4_AAGAAAGAAGATCAGTAAGT
• BKK05980 (tatAY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTGGGCTCCTCCTTT,  downstream forward: _UP4_AAGAAAGAAGATCAGTAAGT

Labs working on this gene/protein

• Jan Maarten van Dijl, University of Groningen, The Netherlands, Homepage

References

Reviews

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Original Publications

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