Rnc

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  • Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

Gene name rnc
Synonyms rncS
Essential yes
Product endoribonuclease III
Function cleaves both 5'- and 3'-sites
of the small cytoplasmic RNA precursor (scr)
Gene expression levels in SubtiExpress: rnc
Metabolic function and regulation of this protein in SubtiPathways:
Rnc
MW, pI 28 kDa, 8.076
Gene length, protein length 747 bp, 249 aa
Immediate neighbours acpA, smc
Sequences Protein DNA DNA_with_flanks
Genetic context
Rnc context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Rnc expression.png















Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU15930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number: 3.1.26.3

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 314 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 605 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References

Reviews


Original publications