FliG

• Description: flagellar basal-body protein, membrane anchor of the basal body, flagellar motor switch protein, physically transduces force from MotA to the rotation of FliF
  
  

Gene name	fliG
Synonyms	flaA15
Essential	no
Product	flagellar motor switch protein
Function	movement and chemotaxis
Gene expression levels in SubtiExpress: fliG
Interactions involving this protein in SubtInteract: FliG
MW, pI	38 kDa, 4.46
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	fliF, fliH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16220

Phenotypes of a mutant

• mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
• no secretion of FlgM, permanent inhibition of SigD PubMed

Database entries

• BsubCyc: BSU16220

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • physically transduces force from MotA to the rotation of FliF

• Protein family: fliG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity: interaction with EpsE prevents energetization of the flagellum by MotA-MotB PubMed

• Interactions:
  • EpsE-FliG PubMed
  • FliF-FliG PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16220

• Structure:

• UniProt: P23448

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon (see there for regulation)
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: fliG PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 250 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: