ThiU

• Description: thiamine ABC transporter (binding protein)
  
  

Gene name	thiU
Synonyms	ykoF
Essential	no
Product	thiamine ABC transporter (binding protein)
Function	thiamine uptake
Gene expression levels in SubtiExpress: thiU
Interactions involving this protein in SubtInteract: ThiU
Metabolic function and regulation of this protein in SubtiPathways: thiU
MW, pI	21 kDa, 6.046
Gene length, protein length	600 bp, 200 aa
Immediate neighbours	thiV, ykoG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU13240

Database entries

• BsubCyc: BSU13240

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ThiW-(ThiV-ThiX)-ThiU PubMed

• Localization: membrane associated (via ThiV-ThiX) PubMed

Database entries

• BsubCyc: BSU13240

• Structure: 1SBR (complex with thiamine), 1S99

• UniProt: O34911

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: thiU-thiV-thiW-thiX PubMed

• Expression browser: thiU PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 257 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 745 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1463 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 433 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 540 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: