YjiB

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Gene name yjiB
Synonyms
Essential no
Product monooxygenase CYP109B1
Function oxidation of fatty acids
Gene expression levels in SubtiExpress: yjiB
Interactions involving this protein in SubtInteract: YjiB
Metabolic function and regulation of this protein in SubtiPathways:
YjiB
MW, pI 44 kDa, 5.379
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours yjiA, yjiC
Sequences Protein DNA DNA_with_flanks
Genetic context
YjiB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YjiB expression.png















Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU12210

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Aili Zhang, Ting Zhang, Emma A Hall, Sean Hutchinson, Max J Cryle, Luet-Lok Wong, Weihong Zhou, Stephen G Bell
The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis.
Mol Biosyst: 2015, 11(3);869-81
[PubMed:25587700] [WorldCat.org] [DOI] (I p)

Marco Girhard, Tobias Klaus, Yogan Khatri, Rita Bernhardt, Vlada B Urlacher
Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 87(2);595-607
[PubMed:20186410] [WorldCat.org] [DOI] (I p)

Marco Girhard, Kazuhiro Machida, Masashi Itoh, Rolf D Schmid, Akira Arisawa, Vlada B Urlacher
Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system.
Microb Cell Fact: 2009, 8;36
[PubMed:19591681] [WorldCat.org] [DOI] (I e)