CdaS

• Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
  
  

Gene name	cdaS
Synonyms	yojJ
Essential	no
Product	sporulation-specific diadenylate cyclase
Function	synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaS
Metabolic function and regulation of this protein in SubtiPathways: CdaS
MW, pI	22 kDa, 5.367
Gene length, protein length	621 bp, 207 aa
Immediate neighbours	yojK, yojI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU19430

Database entries

• BsubCyc: BSU19430

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • synthesis of c-di-AMP from two molecules of ATP PubMed

• Protein family:

• Paralogous protein(s): CdaA

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal autoinhibitory domain PubMed
  • C-terminal DAC domain involved in the synthesis of c-di-AMP PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms hexamers (enzymatically inactive) or dimers (active) PubMed
• Localization:

Database entries

• BsubCyc: BSU19430

• Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)

• UniProt: O31854

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cdaS PubMed

• Expression browser: cdaS PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP983 (cdaS::ermC), available in Jörg Stülke's lab
  • GP1360 (cdaS::spc), available in Jörg Stülke's lab

• Expression vector:
  • IPTG inducible expression of B. cereus Strep-cdaS in E. coli: pGP2593 (in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• His-tag fusion:
  • pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
  • pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab

• two-hybrid system:

• Antibody:

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)