RbsA

• Description: ribose ABC transporter (ATP-binding protein)
  
  

Gene name	rbsA
Synonyms
Essential	no
Product	ribose ABC transporter (ATP-binding protein)
Function	ribose uptake
Gene expression levels in SubtiExpress: rbsA
Interactions involving this protein in SubtInteract: RbsA
Metabolic function and regulation of this protein in SubtiPathways: rbsA
MW, pI	54 kDa, 6.179
Gene length, protein length	1479 bp, 493 aa
Immediate neighbours	rbsD, rbsC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AbrB regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU35940

Database entries

• BsubCyc: BSU35940

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RbsA(2)-(RbsC-RbsD)-RbsB PubMed

• Localization: attached to the cell membrane (via RbsC-RbsD) PubMed

Database entries

• BsubCyc: BSU35940

• Structure:

• UniProt: P36947

• KEGG entry: [3]

• E.C. number: 3.6.3.17

Expression and regulation

• Operon: rbsR-rbsK-rbsD-rbsA-rbsC-rbsB PubMed

• Expression browser: rbsA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • expressed upon transition into the stationary phase (AbrB) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3345 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Le Thi Tam, Christine Eymann, Dirk Albrecht, Rabea Sietmann, Frieder Schauer, Michael Hecker, Haike Antelmann
Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis.
Environ Microbiol: 2006, 8(8);1408-27
[PubMed:16872404] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

M A Strauch
AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon.
J Bacteriol: 1995, 177(23);6727-31
[PubMed:7592460] [WorldCat.org] [DOI] (P p)

K Woodson, K M Devine
Analysis of a ribose transport operon from Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 8);1829-38
[PubMed:7921236] [WorldCat.org] [DOI] (P p)

M O'Reilly, K Woodson, B C Dowds, K M Devine
The citrulline biosynthetic operon, argC-F, and a ribose transport operon, rbs, from Bacillus subtilis are negatively regulated by Spo0A.
Mol Microbiol: 1994, 11(1);87-98
[PubMed:7511775] [WorldCat.org] [DOI] (P p)