rbsK

168

ribokinase

Locus: BSU_35920

Molecular weight: 30.99 kDa

Isoelectric point: 4.61

Protein length: 293 aa

Gene length: 882 bp

Function: ribose utilization

Product: ribokinase

Essential: no

E.C.: 2.7.1.15

Synonyms: rbsK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0524 (Galperin et al., 2021)

Gene

Coordinates: 3,702,393 → 3,703,274

The protein

Catalyzed reaction/ biological activity

• ATP + D-ribose --> ADP + D-ribose 5-phosphate + H⁺ (according to UniProt)

Protein family

• carbohydrate kinase PfkB family (according to UniProt)

Structure

• 1VM7 (PDB) (the enzyme of Thermotoga maritima, 38% identity)
• P36945 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rbsR-rbsK-rbsD-rbsA-rbsC-rbsB

  Description: PubMed

  Regulation

  • expressed upon transition into the stationary phase (AbrB) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • AbrB: activation, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE35920 (ΔrbsK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACCAATCACACAAATATTAC,  downstream forward: _UP4_AATGAAGTAGAGGAGCTGCT
• BKK35920 (ΔrbsK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACCAATCACACAAATATTAC,  downstream forward: _UP4_AATGAAGTAGAGGAGCTGCT

References

Loading