MenB

• Description: naphthoate synthase
  
  

Gene name	menB
Synonyms
Essential	yes PubMed
Product	naphthoate synthase
Function	biosynthesis of menaquinone
Gene expression levels in SubtiExpress: menB
Metabolic function and regulation of this protein in SubtiPathways: menB
MW, pI	29 kDa, 5.343
Gene length, protein length	813 bp, 271 aa
Immediate neighbours	menE, ytxM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU30800

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU30800

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: o-succinylbenzoyl-CoA = CoA + 1,4-dihydroxy-2-naphthoate (according to Swiss-Prot)

• Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-78 and Arg-269 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30800

• Structure: 1RJM (from Mycobacterium tuberculosis, 48% identity, 61% similarity)

• UniProt: P23966

• KEGG entry: [3]

• E.C. number: 4.1.3.36

Expression and regulation

• Operon:
  • menF-menD-ytxM-menB-menE-menC PubMed
  • menB-menE-menC PubMed

• Expression browser: menB PubMed

• Sigma factor:
  • menF: SigA PubMed
  • menB: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 929 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2531 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: