RelA

• Description: GTP pyrophosphokinase (stringent response)
  
  

Gene name	relA
Synonyms
Essential	no
Product	GTP pyrophosphokinase
Function	synthesis and degradation of (p)ppGpp, triggering the stringent response
Gene expression levels in SubtiExpress: relA
Metabolic function and regulation of this protein in SubtiPathways: relA
MW, pI	84 kDa, 6.964
Gene length, protein length	2202 bp, 734 aa
Immediate neighbours	dtd, apt
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU27600

Phenotypes of a mutant

• requirement for valine PubMed
• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed

Database entries

• BsubCyc: BSU27600

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)

• Protein family: relA/spoT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal ppGpp hydrolase domain PubMed
  • central ppGpp synthetase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27600

• Structure:

• UniProt: O54408

• KEGG entry: [3]

• E.C. number: 2.7.6.5

Expression and regulation

• Operon: relA-dtd PubMed

• Expression browser: relA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 244 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 573 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage