ComK

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  • Description: competence transcription factor (CTF)

Gene name comK
Synonyms
Essential no
Product competence transcription factor (CTF)
Function regulation of competence and DNA uptake
Gene expression levels in SubtiExpress: comK
Interactions involving this protein in SubtInteract: ComK
Metabolic function and regulation of this protein in SubtiPathways:
comK
MW, pI 22 kDa, 7.669
Gene length, protein length 576 bp, 192 aa
Immediate neighbours yhzC, yhxD
Sequences Protein DNA DNA_with_flanks
Genetic context
ComK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComK expression.png















Categories containing this gene/protein

genetic competence, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, CodY regulon, ComK regulon, DegU regulon, Rok regulon, Spo0A regulon

The ComK regulon

The gene

Basic information

  • Locus tag: BSU10420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed by Rok PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • induced at the entry to stationary phase (Spo0A-P) PubMed
  • Regulatory mechanism:
  • Additional information:
    • degraded by ClpC-ClpP (with MecA as adaptor protein) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 49 PubMed

Biological materials

  • Mutant:
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

Your additional remarks

References

Reviews

The ComK regulon

Original Publications