PurL

• Description: phosphoribosylformylglycinamidine synthase
  
  

Gene name	purL
Synonyms
Essential	no
Product	phosphoribosylformylglycinamidine synthase
Function	purine biosynthesis
Gene expression levels in SubtiExpress: purL
Interactions involving this protein in SubtInteract: PurL
Metabolic function and regulation of this protein in SubtiPathways: purL
MW, pI	80 kDa, 4.554
Gene length, protein length	2226 bp, 742 aa
Immediate neighbours	purQ, purF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, membrane proteins, most abundant proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

• Locus tag: BSU06480

Database entries

• BsubCyc: BSU06480

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate (according to Swiss-Prot)

• Protein family: FGAMS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • PurS-PurQ-PurL PubMed

• Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed

Database entries

• BsubCyc: BSU06480

• Structure: 3D54 (from Thermotoga maritima, 39% identity, 58% similarity) PubMed

• UniProt: P12042

• KEGG entry: [3]

• E.C. number: 6.3.5.3

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD PubMed

• Expression browser: purL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • repressed in the presence of guanine(G-box) PubMed

• Regulatory mechanism:
  • [PurR]]: transcription repression PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed1 PubMed2

• Additional information:
  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3495 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 10654 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yanfei Xia, Shanshan Xie, Xin Ma, Huijun Wu, Xuan Wang, Xuewen Gao
The purL gene of Bacillus subtilis is associated with nematicidal activity.
FEMS Microbiol Lett: 2011, 322(2);99-107
[PubMed:21671997] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

Aaron A Hoskins, Ruchi Anand, Steven E Ealick, JoAnne Stubbe
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.
Biochemistry: 2004, 43(32);10314-27
[PubMed:15301530] [WorldCat.org] [DOI] (P p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807] [WorldCat.org] (P p)