Btr

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  • Description: transcriptional activator (AraC family), (AraC family DNA-binding domain fused to FeuA-like substrate-binding domain), regulation of the feuA-feuB-feuC-ybbA operon

Gene name btr
Synonyms ybbB, yzbC
Essential no
Product transcription activator (AraC family)
Function regulation of iron acquisition
Gene expression levels in SubtiExpress: btr
Metabolic function and regulation of this protein in SubtiPathways:
btr
MW, pI 60 kDa, 6.433
Gene length, protein length 1587 bp, 529 aa
Immediate neighbours feuA, ybbC
Sequences Protein DNA DNA_with_flanks
Genetic context
YbbB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Btr expression.png















Categories containing this gene/protein

acquisition of iron, iron metabolism, transcription factors and their control

This gene is a member of the following regulons

Fur regulon

The Btr regulon:

The gene

Basic information

  • Locus tag: BSU01640

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binding of a direct repeat in the feuA promoter region and transcription activation of the feuA-feuB-feuC-ybbA operon in the presence of bacillibactin or enterobactin PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): enterobactin, bacillibactin (co-activators) PubMed
  • Effectors of protein activity: enterobactin, bacillibactin (co-activators) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed under conditions of iron starvation (Fur) PubMed
  • Regulatory mechanism:
  • Additional information:
    • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Semen A Leyn, Marat D Kazanov, Natalia V Sernova, Ekaterina O Ermakova, Pavel S Novichkov, Dmitry A Rodionov
Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis.
J Bacteriol: 2013, 195(11);2463-73
[PubMed:23504016] [WorldCat.org] [DOI] (I p)

Letal I Salzberg, Leagh Powell, Karsten Hokamp, Eric Botella, David Noone, Kevin M Devine
The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells.
Mol Microbiol: 2013, 87(1);180-95
[PubMed:23199363] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Shawn MacLellan, John D Helmann
Transcription activation by the siderophore sensor Btr is mediated by ligand-dependent stimulation of promoter clearance.
Nucleic Acids Res: 2012, 40(8);3585-95
[PubMed:22210890] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, John D Helmann
Substrate induction of siderophore transport in Bacillus subtilis mediated by a novel one-component regulator.
Mol Microbiol: 2007, 66(1);164-73
[PubMed:17725565] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol Microbiol: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)