AbfA

• Description: alpha-L-arabinofuranosidase
  
  

Gene name	abfA
Synonyms
Essential	no
Product	alpha-L-arabinofuranosidase
Function	arabinan degradation
Gene expression levels in SubtiExpress: abfA
Metabolic function and regulation of this protein in SubtiPathways: abfA
MW, pI	56 kDa, 5.344
Gene length, protein length	1500 bp, 500 aa
Immediate neighbours	cstA, araQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AraR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU28720

Database entries

• BsubCyc: BSU28720

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 51 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • forms hexamers PubMed

Database entries

• BsubCyc: BSU28720

• Structure: 1QW8 (complex with Ara-alpha-Xyl, Geobacillus stearothermophilus), 1PZ3 (Geobacillus stearothermophilus)

• UniProt: P94531

• KEGG entry: [3]

• E.C. number: 3.2.1.55

Expression and regulation

• Operon: araA-araB-araD-araL-araM-araN-araP-araQ-abfA PubMed

• Expression browser: abfA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: