RapF

From SubtiWiki
Revision as of 15:05, 2 April 2014 by 134.76.38.147 (talk)
Jump to: navigation, search
Gene name rapF
Synonyms ywhJ
Essential no
Product response regulator aspartate phosphatase
Function control of ComA activity
Gene expression levels in SubtiExpress: rapF
Interactions involving this protein in SubtInteract: RapF
MW, pI 45 kDa, 5.18
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours ywhK, phrF
Sequences Protein DNA DNA_with_flanks
Genetic context
RapF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RapF expression.png
























Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

CcpA regulon, ComA regulon

The gene

Basic information

  • Locus tag: BSU37460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: control of ComA activity PubMed
  • Protein family: RAP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

Database entries

  • Structure:
    • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: repressed by glucose (5-fold) (CcpA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References