FolE2

• Description: GTP cyclohydrolase IB, replaces FolE under conditions of zinc starvation
  
  

Gene name	folE2
Synonyms	yciA, folEB
Essential	no
Product	zinc-independent GTP cyclohydrolase IB
Function	biosynthesis of folate
Gene expression levels in SubtiExpress: folE2
MW, pI	34 kDa, 7.831
Gene length, protein length	915 bp, 305 aa
Immediate neighbours	nasA, yciB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Zur regulon

The gene

Basic information

• Locus tag: BSU03340

Database entries

• BsubCyc: BSU03340

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: GTP + H₂O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate (according to Swiss-Prot)

• Protein family: GTP cyclohydrolase IV family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): activated by a variety of divalent cations PubMed

• Effectors of protein activity:

• Interactions: homotetrameric PubMed

• Localization:

Database entries

• BsubCyc: BSU03340

• Structure: 3D1T PubMed

• UniProt: P94398

• KEGG entry: [3]

• E.C. number: 3.5.4.16

Expression and regulation

• Operon: folE2-yciB-yciC PubMed

• Expression browser: folE2 PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by zinc starvation (Zur) PubMed

• Regulatory mechanism:
  • Zur: transcription repression in the presence of zinc PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: