OpuD

• Description: glycine betaine transporter
  
  

Gene name	opuD
Synonyms	ytfQ
Essential	no
Product	glycine betaine transporter
Function	compatible solute transport
Gene expression levels in SubtiExpress: opuD
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	55 kDa, 9.508
Gene length, protein length	1536 bp, 512 aa
Immediate neighbours	ytfP, yteV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, coping with hyper-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU30070

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54417

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: opuD PubMed

• Sigma factor: SigA PubMed , SigB PubMed

• Regulation:
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A1052 ( opuD::kan), PubMed, available at BGSC
  • 1A1053 ( opuD::kan), PubMed, available at BGSC
  • 1A1054 ( opuD::kan), PubMed, available at BGSC
  • 1A1055 ( opuD::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

R M Kappes, B Kempf, E Bremer
Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD.
J Bacteriol: 1996, 178(17);5071-9
[PubMed:8752321] [WorldCat.org] [DOI] (P p)